From single molecules to whole organisms, 3i LightSheet systems enable live cell imaging beyond the limitations of traditional confocal modalities
Axially Swept Light Sheet Microscopy System for Imaging Cleared Specimens
AxL Cleared Tissue LightSheet (AxL CTLS) is a fully automated macro zoom microscope with high NA apochromatic objectives and dual-sided light sheet illumination for imaging whole organs to small animals. Custom-designed excitation objectives and patented axially swept light sheet microscopy (ASLM) produce an exceptionally thin, long and uniform lightsheet for large-scale high-resolution imaging. Using a back-thinned sCMOS camera, AxL CTLS images one cubic centimeter in less than a minute.
Brain Vasculature
Smooth muscle cells in the arteries, veins, and capillaries of mouse brain cleared with PEGASOS labeled by NG2BacDsRed. Imaged by Cleared Tissue LightSheet. Sample courtesy of Dr. Woo-Ping Ge, University of Texas Southwestern Medical Center.
A Microscope for High-Resolution, Fast, and Gentle 3D Live Cell Imaging
Lattice LightSheet (LLS) is a cutting-edge light sheet microscope built for cell biologists. The system generates an optical lattice to create an ultra-thin light sheet to image biological samples over long periods of time and with very fine resolution. This allows for 4D live cell imaging, where experiments limited to seconds or minutes on other imaging platforms can be extended to hours or even days. The combination of high spatiotemporal resolution, imaging speed and sensitivity make Lattice LightSheet the ultimate imaging tool for a new era of live cell microscopy.
Hela cells
HeLa cells transfected with GFP-Lifeact (488nm) to visualize actin dynamics and stained with DiL (561nm red)(Thermo) to visualize membrane dynamics. Courtesy of James Springfield, Institute for Molecular Bioscience (IMB) at the University of Queensland
Versatile Multiview Light Sheet Microscopy System for Imaging Model Organisms
Marianas LightSheet (MLS) combines the low phototoxicity and large sample space of dual inverted selective plane illumination microscopy (diSPIM) with the power and flexibility of a research-grade inverted microscope system. MLS supports a wide variety of specimen types and sizes as well as experimental designs including photoablation, photomanipulation, and computer-generated holography. Add spinning disk confocal, TIRF and FLIM to transform the system into a versatile multimodal imaging system all controlled with SlideBook software.
